Edwin
Broun Fred and Selman A. Waksman, Laboratory
Manual of General Microbiology (New York:
McGraw-Hill, 1928).
In the early 1920s, a generation of microbiologists
inspired by the work of Sergei Winogradsky and
others, strove to make soil microbiology an
independent scientific discipline. As such,
it would need its own journals, institutions,
and experimental methods. To satisfy that latter,
in 1928, Fred and Waksman produced this laboratory
manual for students of General Microbiology
who were interested in working with soils and
organisms isolated from the soil. They offered
methods to isolate and cultivate bacteria, fungi,
actinomyces, algae, and protozoa (the organisms
associated with soil processes related to agriculture).
They paid special attention to the physiology
of these organisms and its relationship to the
transformation carbon, nitrogen, phosphorous
and sulphur.
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